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Version: 2.0
Template Description: Template for Genotyping Data using EcotillingTemplate for Genotyping Data using Ecotilling
Mappings for this template
Sections available in this template
| Section Name | Description | Conditions |
| Source | Information on the source of the dataset, the species it concerns and the name and version of the dataset | Mandatory
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| Experiment | General experiment data | Mandatory
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| Quality Assessment | Information about the quality measures used | Mandatory
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| Conditions | Experimental conditions | Mandatory
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| Data List | The actual data as a list | Mandatory
Multiple sheets allowed
Excludes Data Matrix
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| Data Matrix | The actual data as a matrix | Mandatory
Multiple sheets allowed
Excludes Data List
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| Markers | Information about markers used in the experiment | Optional
Multiple sheets allowed
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| Samples | Information about accessions used in the experiment | Mandatory
Multiple sheets allowed
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| Institutions | List of institute codes used in passport data sections and their corresponding decoded name and addresses. | Optional
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Source
Section Description: Information on the source of the dataset, the species it concerns and the name and version of the dataset
see section: source in GCPDataSubmissionTemplate2.0
for the following fields
institute, principalInvestigator, projectCode, projectName, emailContact, species, ploidy, datasetName, version, creationDate, remark
Experiment
Section Description: General experiment data
see section: experiment in GCPGenotypingTemplate2.0
for the following fields
operationalTaxonomicUnit, purposeOfStudy, missingData, remark
Quality Assessment
Section Description: Information about the quality measures used
see section: qualityAssessment in GCPDataSubmissionTemplate2.0
for the following fields
qualityMeasure, standard, control, errorEstimator
Conditions
Section Description: Experimental conditions
see section: conditions in GCPGenotypingTemplate2.0
for the following fields
samplingStrategy, controlGenotypes, sizeStandard, dnaExtraction, dnaAmplificationDetection, scoringMethod, genotypingSoftware, reference
| Field Name | Description | Conditions |
| Scoring Method | Describe the method used for scoring the gels in the event that a genotyping software is not used. | Mandatory
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Data List
Section Description: The actual data as a list
| Field Name | Description | Conditions |
| Sample ID | A unique identifier of a DNA sample, which can be a sample in a well on a gel or a LIMS entry, or even a unique ID created specifically for this dataset. The SampleID is specific to a lab and is not a universal identifier. If the accession data is provide it must relate to SampleID in the accession sheet or file. | Mandatory
Unique
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| Germplasm ID | A unique alphanumeric value which identifies the germplasm. This global identifier links data across domains. The format proposed is concatenation of holdingInstitute:collectionName:localUniqueID. | Mandatory
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| Locus | The name of the locus used as a marker. If the marker data is provide it must relate to Marker name in the marker sheet or file. | Mandatory
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| Reference Sample | The name or number of the gel or gel run from which the data was taken. This gel name will be unique for a specific laboratory but is not a universal identifier. In the case of the ABI sequencer this may be the run number. | Optional
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| Gel/Run | The name or number of the gel or gel run from which the data was taken. This gel name will be unique for a specific laboratory but is not a universal identifier. In the case of the ABI sequencer this may be the run number. | Optional
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| Dye | The dye used for detection of the peak. | Optional
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| Allele | The allelic band observed. This value depends on the scoring scheme used, which is described in Section: Conditions.
Example: 1
Example: 0 | Mandatory
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Data Matrix
Section Description: The actual data as a matrix
| Field Name | Description | Conditions |
| Sample ID | A unique identifier of a DNA sample, which can be a sample in a well on a gel or a LIMS entry, or even a unique ID created specifically for this dataset. The SampleID is specific to a lab and is not a universal identifier. If the accession data is provide it must relate to SampleID in the accession sheet or file. | Mandatory
Unique
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| Germplasm ID | A unique alphanumeric value which identifies the germplasm. This global identifier links data across domains. The format proposed is concatenation of holdingInstitute:collectionName:localUniqueID. | Mandatory
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| Alleles | none | Mandatory
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Markers
Section Description: Information about markers used in the experiment
see section: markers in GCPMappingTemplate2.0
for the following fields
marker, genBankAccessionNumber, forwardPrimer, reversePrimer, annealingTm, minAllele, maxAllele, references
Samples
Section Description: Information about accessions used in the experiment
Samples (optional)
The first field in the sample is the SampleID, which relates directly to the SampleID field in the data spreadsheet or file. This SampleID is a unique identifier of a DNA sample, which can be a sample in a well on a gel or a LIMS entry. It could even by a unique identifier developed specifically for this dataset. In the case of multiple extractions from the same material then each same would have a unique SampleID. Please refer to the section on Multiple Data Points for more details.
The GermplasmID field is an optional field for collections where a new GermplasmID is assigned each time an accession is regenerated or for some other reason a new seed or germplasm sample is taken. For this reason an accession in this case is a collection of samples with different GermplasmIDs. GermplasmID are often unique within a specific database for this reason they should be prefixed by the data name or abbreviation. For example, an entry with GermplasmID 2341 in IWIS, would be IWIS:2341.
The remaining accession data should be either in multi-crop passport descriptors (MCPD) or EURISCO descriptors format. These descriptors are MCPD defines a total of 28 descriptors for passport data, each of which equates to a column in the template. EURISCO defines an additional 6 descriptors for a total of 33 descriptors. Only a few MCPD or EURISCO descriptors are mandatory and for the sake of brevity only the mandatory and some recommended optional fields are described here. However, the mandatory descriptor provides sufficient information to allow the accession to be found in the appropriate National Inventory or genebank. For a full description of all MCPD and EURISCO descriptors please refer to the EURISCO_Descriptors.doc file, which is available fro the EPGRIS website (http://www.ecpgr.cgiar.org/epgris/) and or can be downloaded with the passport template.
see section: generalPassportData in GCPPassportTemplate2.0
for the following fields
sampleID, sampleGermplasmID, localUniqueID, holdingInstitute, collectionName, genus, species, countryOfOrigin
| Field Name | Description | Conditions |
| Sample ID | A unique identifier of a DNA sample, which can be a sample in a well on a gel or a LIMS entry. The SampleID will be unique for a specific laboratory but is not a universal identifier. It must relate to SampleID in the data spreadsheet or file. | Mandatory
Unique
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| Germplasm ID | A alphanumeric value which uniquely identifies the germplasm. The format proposed is concatenation of HoldingInstitute:CollectionName:LocalUniqueID. In case a new Germplasm ID is assigned each time an accession is regenerated or for some reason sub-sampled use the current germplasm ID prefixed with the system or database name.
Example: NGA333:Genebank:252
Example: COL003:CIATBEAN:3542
Example: MEX064:IWIS:2341 | Mandatory
Unique
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| Country of Origin | Code of the country in which the sample was originally collected. Use 3-letter ISO 3166-1 extended country codes. | Optional
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Institutions
Section Description: List of institute codes used in passport data sections and their corresponding decoded name and addresses.
see section: institutions in GCPDataSubmissionTemplate2.0
for the following fields
faoInstituteCode, organizationName, street, cityState, zipCode, country, institutionalEmail, institutionalTelephone, fax, url, primaryContactName
Copyright (c) 2004-2006 CIMMYT, CIRAD, IRRI, IRRI, IRRI
Developed by Guy Davenport (CIMMYT), Claire Billot (CIRAD), Kenneth McNally (IRRI), Ma. Elizabeth Naredo (IRRI), Genevieve Aquino (IRRI)
This work is licensed under a Creative Commons Attribution-ShareAlike 2.5 License.
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